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The eMOTIONAL Cities research focusing on the signals triggered in our neurobiological architecture, responsible for emotions and decisions, while humans interact with the urban environment, will shed light on how to improve population health, physical and/or mental. Adopting a systematic approach, based on natural experiments and actual problems of case-study cities, eMOTIONAL Cities will provide robust scientific evidence on how the natural and built urban environment shapes human cognitive and emotional processing, with a perspective that also incorporates age, gender and vulnerable groups’ specificities – such as elderly subjects with mild cognitive impairment.